Fig. 4.

Renal cold storage followed by transplantation (CS/Tx) alters mitochondrial respiratory complex protein homeostasis in rat kidneys. A: Triton-soluble fraction. B: Triton-insoluble fraction. C: total extracts were prepared from rat kidney homogenates from control (untreated), cold storage (CS; 18-h), sham (1-day reperfusion post-right nephrectomy), ATx (no CS; 1-day reperfusion posttransplant), or CS/Tx (18-h CS plus 1-day reperfusion posttransplant). Renal extracts (30 µg) from Triton-soluble and Triton-insoluble fractions and total extracts were resolved on SDS-PAGE gel followed by Western blotting for SDHA and ATP5B. Representative blots of 3 experiments are shown. X in B indicates empty lane. Densitometry values were normalized to the β-actin loading control and are expressed as relative abundance of control (for CS) or sham (for ATx and CS/Tx; bar graphs, n = 3). Differences between groups were compared with a Student’s t-test (control vs. CS) or 1-way ANOVA with Tukey’s post hoc test for multiple group comparisons (sham, ATx, and CS/Tx). *Means are significantly different (P < 0.05) between sham and CS/Tx. #Means are significantly different (P < 0.05) between ATx and CS/Tx.