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. 2019 Feb 20;16:23. doi: 10.1186/s12985-019-1130-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of polyadenylation on in vitro translation mediated by WYMV UTRs. a Constructs of firefly luciferase reporter vector containing UTRs of WYMV RNA1. b Effect of UTRs of WYMV RNA1 with or without poly(A) on translation of F-luc. c Constructs of firefly luciferase reporter vector containing UTRs of WYMV RNA2. d Effect of UTRs of WYMV RNA2 with or without poly(A) on translation of F-luc. 5’UTR and 3’UTR of WYMV RNA1 (AF067124) is 162 nt and 258 nt, respectively; 5’UTR and 3’UTR of WYMV RNA2 (AF041041) is 171 nt and 768 nt, respectively. F-luc indicates firefly luciferase coding region; shadow boxes indicate corresponding WYMV UTRs