Fig. 1.

Chlamydia pneumoniae infects human astrocytes in vitro. STTG1 human astrocytes infected with Cpn strain AR39 at an MOI of 1 demonstrated a diffuse punctate labeling of Cpn (green) from 6 to 72 hpi. Nuclei are labeled with DAPI (blue). Scale bar represents 20 μm (a). Infected versus uninfected cell counts were averaged across approximately N = 2000–2500 cells per timepoint and in biological triplicate across two independent infections. Numerical data is expressed as percent infected cells (b). Percentages of infected cells were significantly different between 6 hpi versus 48 hpi and 72 hpi, 24 hpi versus 48 hpi and 72 hpi. Comparisons between populations were determined through one-way ANOVA, where significance was defined as p < 0.05, and confirmed using Tukey HSD post hoc analysis. Error bars represent standard deviation of the mean