Fig. 3.

Chlamydia pneumoniae infection of astrocytes alters labeling of secretases. Astrocytes infected with Cpn from 6 to 72 hpi were double labeled for Cpn (green) and secretases ADAM10, BACE1 or PSEN1 C-terminal fragment (red). 10–15 cells per biological replicate were imaged against an equal number of uninfected control cells. Cells were visualized using laser scanning confocal microscopy, maintaining the voltage settings of each color channel identical across biological replicates. DAPI was used to visualize the nucleus. Representative images are included this figure. Scale bar represents 20 μm