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. 2019 Feb 20;20:6. doi: 10.1186/s12868-019-0489-5

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Fluorescence intensity of Aβ_1-42 is increased in Chlamydia pneumoniae-infected astrocytes. Aβ_1-42 (red) and Cpn (green) were visualized by laser scanning, confocal microscopy (a). To analyze the Z-images using FIJI software, a defined threshold subtraction was applied equally to each image to determine Aβ_1-42 fluorescence intensity (b); mean fluorescence intensity was calculated for infected and uninfected astrocytes at 24, 48 and 72 hpi. Cells (N = 25–30) were analyzed across three biological replicates to reliably conduct a student’s t-test on the fluorescence intensities of Aβ_1-2 in uninfected and Cpn-infected cells. Error bars represent standard error of the mean. Asterisk represents p < 0.05