Fig. 6.

Quantification of sAPPβ/total sAPP in media of uninfected and Chlamydia pneumoniae infected astrocytes using MSD ELISA. Conditioned media of uninfected and Cpn-infected cells at each timepoint post-infection was collected, concentrated, and assayed in equal volumes for sAPPβ and sAPPα levels. Standard curves of known concentrations of sAPPβ and sAPPα was used to determine the concentration of these individual Aβ species. Conditioned media was obtained from three biological replicates and the assay was conducted in technical triplicate. Student’s t-test was calculated using the average sAPPβ/total sAPP ratio of Cpn-infected conditioned media compared to that of uninfected conditioned media. Asterisk represents p < 0.05