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. 2019 Feb 20;20:6. doi: 10.1186/s12868-019-0489-5

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — BACE1 activity is increased and ADAM10 activity is decreased in Chlamydia pneumoniae infected astrocytes. FRET-based assays were used to quantify the activity of ADAM10 and BACE1 enzyme activity generated by Cpn-infected and uninfected cell lysates at 48 hpi. Fluorescence of the 5-FAM or HiLyte Fluor 488 dyes conjugated to ADAM10 or BACE1-specific substrates was detected as a result of cleavage by the respective enzymes and compared back to fluorescence intensity of known dye concentrations. The quantified dye concentration from ADAM10 and BACE1 fluorescent substrate cleavage from (N = 4) biological replicates of Cpn-infected and uninfected cell lysates is presented as an average in the histograms. Error bars represent standard error of the mean. Asterisk represents p < 0.05