Table 1.
Sequences for primers
| Gene (Primers) | Sequences |
|---|---|
| TNF-α | |
| Forward primer | 5′-CCTTATCTACTCCCAGGTTCTC-3′ |
| Reverse primer | 5′-TTTCTCCTGGTATGAATGGC-3′ |
| IL-1β | |
| Forward primer | 5′-CGACAGAATCTAGTTGTCC-3′ |
| Reverse primer | 5′-TCATAAACACTCTCATCCACAC-3′ |
| COX-2 | |
| Forward primer | 5′-GGCACAAATATGATGTTCGCA-3′ |
| Reverse primer | 5′-CCTCGCTTCTGATCTGTCTTGA-3′ |
| AT1aR | |
| Forward primer | 5′-ACTCACAGCAACCCTCCAAG-3′ |
| Reverse primer | 5′-ATCACCACCAAGCTGTTTCC-3′ |
| ACE | |
| Forward primer | 5′-GTGTTGTGGAACGAATACGC-3′ |
| Reverse primer | 5′-CCTTCTTTATGATCCGCTTGA-3′ |
| ACE2 | |
| Forward primer | 5′-GGCTCCTTCTCAGCCTTG-3′ |
| Reverse primer | 5′-TTCATAAAAGGCAGACCATTTG-3′ |
| AVP | |
| Forward primer | 5′-TGCCTGCTACTTCCAGAACTGC-3′ |
| Reverse primer | 5′-AGGGGAGACACTGTCTCAGCTC-3′ |
| GAPDH | |
| Forward primer | 5′-AAGGTCATCCCAGAGCTGAA-3′ |
| Reverse primer | 5′-ATGTAGGCCATGAGGTCCAC-3′ |
TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; COX-2, cyclooxygenase-2; AT1aR, angiotensin II type 1a receptor; ACE, angiotensin-converting enzyme; AVP, arginine vasopressin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.