Figure 7.

Functional TRPVs influenced melanoma cell fate in different profiles. (a) Flow cytometry assessment of 4α-PDD triggered apoptosis (i) of melanoma A375 cells via staining with FITC - Annexin V and PI. Activation of TRPV4 by 4α-PDD induced human melanoma A375 cells undergoing early apoptosis (ii). A slight degree of necrotic cells was observed as well (iii). (b) Flow cytometry assessment of 2-APB triggered cell death of melanoma A2058 cells via staining with Hochest33342 and PI. Activation of TRPV2 by 2-APB in melanoma A2058 cells underwent late apoptosis as well as necrosis (i). 2-APB application induced a slight degree of early apoptotic cells (ii) and dominated cells undergoing necrosis and late apoptosis (iii).