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Effects of RVSE on proliferation and cell cycle arrest in MCF-7 cells. (a) Cytotoxicity of RVSE on MCF-7 cells (1 × 104/well) by MTT assay. (b) Cells (5 × 105/mL) were treated with 200–400 μg/mL RVSE for 24 h, fixed, stained with PI, and analyzed by flow cytometry. (c) Percentage of cells in sub-G1 phase. Data are means ± SD of three independent experiments. ∗P < 0.05 and ∗∗P < 0.01, significant difference from the control.
