Figure 5.

Effects of resveratrol and PGC1α siRNA on HG-induced total ROS production and mitochondrial injury in podocytes. (A, A1) Intracellular ROS was detected by flow cytometry. (B) The mitochondrial ROS production in podocytes was detected by flow cytometry. (C) The changes of mitochondrial complex I and III activities in podocytes were detected by kit. (D) ΔΨYm was measured using a fluorescent probe JC-1. The ratio of red/green fluorescence represented ΔΨm in podocytes. The values are expressed as mean ±SD. ^aa P<0.01 vs. normal-glucose group; * P<0.05, ** P<0.01 vs. high-glucose group; ^## P<0.01 vs. high-glucose + resveratrol group.