Figure 2.

Knockdown of POU5F1B inhibited proliferation and viability in cervical cancer cells. (A) POU5F1B expression was examined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) in cervical cancer cells transfected with si-POU5F1B or si-NC. (B) The effects of POU5F1B knockdown on cell proliferation were analyzed by the Cell Counting Kit-8 (CCK-8) assay. Colonies were counted and the images were captured. (C, D) The effects of POU5F1B knockdown on cell proliferation, analyzed by colony formation assays. (E, F) The effects of POU5F1B knockdown on cell proliferation was analyzed by EDU proliferation assays, The he Click-it reaction showed EdU staining (red). Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (blue). The experiments were performed in triplicate ±SD. The values are considered significant at * P<0.01, ** P<0.01.