Fig. 1.

Pyruvate dehydrogenase activity (PDHa) expressed as a % of total PDH activity (PDHt) in rat gastrocnemius-plantaris muscle at rest, during 0.5 Hz or 1.0 Hz stimulation, and after dichloroacetate (DCA) treatment at rest. Freeze-clamped muscle samples were collected from anesthetized Wistar rats and assayed in vitro for PDH activity via radioisotopic assay described in materials and methods. Values are means ± SE for resting (n = 5) and 0.5 Hz (n = 4), 1.0 Hz (n = 4), and DCA (n = 4) treatment groups. *Significantly different from resting (P < 0.05); †significantly different from 0.5 Hz (P < 0.05). Significance determined by 2-tailed Student’s t-test.