Table 1.

Resting metabolite, pH, and ΔG_ATP before and after DCA treatment

	Control (n = 6)	DCA (n = 6)
Pi, mmol/LCW	1.61 ± 0.11	1.34 ± 0.16*
PCr, mmol/LCW	38.0 ± 1.21	37.2 ± 1.63
ATP, mmol/LCW	9.24 ± 0.7
PCr/ATP	4.01 ± 0.13	3.92 ± 0.18
pH	7.01 ± 0.02	6.98 ± 0.01
ΔGATP, kJ/mol	−65.7 ± 0.22	−66.4 ± 0.30*

Values are means ± SE; n = 6 per group. Resting values were calculated from high-resolution spectra as seen in Fig. 2. Peak areas were quantified with NUTS line-fitting algorithm and normalized to total ³¹P peak area for each spectrum. Spectra were quantified with γ-ATP peak area at ATP concentration of 9.24 mM determined enzymatically in control white gastrocnemius muscle. pH was calculated via chemical shift of inorganic phosphate (P_i) relative to phosphocreatine (PCr) as shown in Eq. 1. Free energy of ATP hydrolysis (ΔG_ATP) was calculated as described in Eq 3. DCA, dichloroacetate; LCW, liter cell water.

^*Significant difference between control and DCA conditions (P < 0.05) by 2-tailed paired Student’s t-test.