Fig. 8.
Effect of chronic LPS exposure on T-regulatory cells (Tregs) of saccular lungs. Newborn mice were treated intraperitoneally with LPS at doses of 3 (L3), 6 (L6), or 10 (L10) mg/kg or a vehicle control (PBS) on postnatal days (PNDs) 3–5, and the lung tissues were collected on PND6 to quantify CD4+TCRβ+ (A) and CD4+TCRβ+FoxP3+ (Tregs; B) cells using flow cytometry. The data shown are representative of two independent experiments. Values are presented as means ± SD (n = 6/group). Significant differences between PBS- and LPS-treated animals are indicated by ***P < 0.001, **P < 0.01, and *P < 0.05. Significant differences between L3- and L10-exposed animals are indicated by §§P < 0.01. Significant differences between L3- and L6-exposed animals are indicated by †P < 0.05 (one-way ANOVA).