Fig. 1.

Transforming growth factor-β (TGFβ) decreases soluble guanylate cyclase-α1 (sGCα1) mRNA expression in pulmonary artery smooth muscle cells (PASMC). Serum-restricted CS54 cells were treated with either none or the indicated amounts of recombinant TGFβ-1 for the times shown. Subsequently, sGCα1 mRNA levels relative to that of GAPDH were determined in cell lysates using specific primers and quantitative PCR, and the %inhibition of sGCα1 mRNA expression was determined. Open bars, n = 3 in each group; gray bars, data combined from the cells treated with 2.5–20 ng/ml TGFβ-1 for the indicated times. *P < 0.015 vs. the other time intervals.