Table 5.

MUC1 HLA-A3 and HLA-A24 agonist epitope-specific T-cell lines produce Type I cytokines upon stimulation

T-cell line	Peptide	IFN-γ	GM-CSF	IL-2	TNFα	IL-8	IL-6	IL-10
A3
T-C4	C4A	2,777	1,135	4	119	20	71	27
T-C4A	C4A	>10,000	>10,000	170	3,804	279	192	79
T-C5	C5A	>10,000	6,355	347	2,576	165	138	16
T-C5A	C5A	>10,000	>10,000	1,266	7,546	2,738	414	74
A24
T-C7	C7	750	237	<2.4	21	6.8	6.4	25
	C7A	1,279	300	<2.4	30	7.3	7.7	45
T-C7A	C7	680	215	<2.4	30	112	<2.4	92
	C7A	2,000	910	<2.4	70	360	40	375

The MUC1 specific T-cell lines were evaluated for cytokine production upon stimulation with autologous B cells pulsed with the corresponding native and agonist peptides. For the HLA-A3 peptide epitopes, T-cell lines were established from PBMCs from a patient with colon cancer treated with PANVAC vaccine. The T-cell lines were used at IVS5 (T-C4 and T-C4A) and IVS4 (T-C5 and T-C5A), stimulated with B cells pulsed with the corresponding peptides for 24 h, and cytokine levels were analyzed in the supernatant. 1 × 10⁶ T cells/ml, values expressed as pg/ml. For the HLA-A24 peptide epitopes, T-cell lines were established from PBMCs from a patient with prostate cancer treated with PSA-TRICOM and ipilimumab. Despite several attempts, it was not possible to establish a T-cell line using the native C6 peptide, although a T-cell line could be established from the same patient with the corresponding agonist peptide. The native T-C7 and agonist T-C7A T-cell lines were stimulated for 24 h with B cells pulsed with both the native and the agonist peptides, and cytokine levels in the supernatant were analyzed. Results are expressed as pg/ml/2.5 × 10⁵ T cells. The levels of IL-12p70 and IL-1β were < 100 pg/ml for all native and agonist epitopes