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. Author manuscript; available in PMC: 2019 Jun 6.
Published in final edited form as: Neuron. 2018 May 10;98(5):992–1004.e4. doi: 10.1016/j.neuron.2018.04.030

Figure 2: ChR2-based optogenetic stimulation of serotonergic neurons using ePet1-cre;Ai32 mice.

Figure 2:

Schematic for intracellular recordings (IC) in raphe containing brain slices of serotonergic neurons in the DR (a) and MR (e). Relationship between the frequency of stimulation using 473 nm pulses of light and the firing frequency of ChR2-YFP expressing DR (b) and MR (f) cells in acute brainstem slices. Vertical blue shading indicates optogenetic stimulation. Schematic for in vivo photostimulation of serotonergic neurons in the DR (c) and MR (g). ePet1-cre;Ai32 mice displayed hyperlocomotion when stimulated using blue light in the DR (d) or MR (h) (blue band; 473nm, 10ms, 20Hz). Control: ePet1-cre−/−;Ai32+/+; ChR2: ePet1-cre+/−;Ai32+/+. Posthoc genotype effect in three minute light on block is indicated; **: p < 0.01, ***: p < 0.001.