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. 2019 Feb 7;21(1):51–60. doi: 10.1089/cell.2018.0050

Table 2.

In Vitro Development of Embryos Cloned Using Different Methods

Group No. of cultured zygotes No. of cloned oocytes No. (%) of fused embryos* No. (%) ≥2–4 cell embryos** No. (%) ≥8–16 cell embryos** No. (%) of blastocysts***
IVF 258 219 (85.0 ± 1.5)b 194 (75.4 ± 1.3)a 80 (31.1 ± 1.1)a
SCNT 558 381 (68.3 ± 1.5)a 303 (79.5 ± 1.4)a 148 (39.7 ± 2.1)b 74 (20.2 ± 1.3)b
CICT 296 243 (82.0 ± 0.3)b 200 (82.0 ± 1.7)ab 149 (61.5 ± 1.3)c 70 (28.9 ± 0.8)a

Mean ± standard error of the mean.

a–c

Values with different superscripts in the same column are significantly different (p < 0.05).

*

Fusion rates were calculated based on the number of injected oocytes.

**

Cleavage rates were calculated based on the number of fused embryos for SCNT, CICT and based on the number of fertilized zygote for IVF.

***

Blastocyst development rates were calculated based on the number of fused embryos for SCNT and CICT and based on the number of presumed zygote for IVF.

SCNT, somatic cell nuclear transfer; CICT, cytoplasm injection cloning technology; IVF, in vitro fertilization.