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. 2019 Feb 5;38(2):176–183. doi: 10.1089/dna.2018.4462

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© Guodong Ma et al., 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 3. — LINC00460 regulates the EGFR protein through sponging miR-769-5p. (A) EGFR mRNA expression was measured in the gefitinib chemotherapy resistance of NSCLC cells (A549/GR) and A549 cells transfected with siRNA and plasmids. (B) EGFR mRNA expression was measured in the gefitinib chemotherapy resistance of NSCLC cells (A549/GR) and A549 cells. (C) Subcellular fractionation analysis showed the distribution of LINC00460 in the cytoplasm. (D) Schematic diagram for the LINC00460 3′-UTR and miR-769-5p. Luciferase assay was performed to confirm it. (E) miR-769-5p expression was measured using PCR in the A549/GR cells transfected with siRNA-LINC00460. (F) Schematic diagram for the EGFR 3′-UTR and miR-769-5p. Luciferase assay was performed to confirm it. (G) EGFR mRNA expression was measured in A549/GR cells transfected with miR-769-5p mimics. Data are expressed as mean ± SD. *p < 0.05, **p < 0.01 represents statistical difference. EGFR, epidermal growth factor receptor.