Table 2.
Specificity of the interaction of steroids 3,4,6,9 with other ABC transporters.
| Modulators (10 µM) | K562/R7 cells (P-gp)a | HL60-MRP1 cells (MRP1)a | MCF7-MTX cells (BCRP)b |
|---|---|---|---|
| 3 | 2.5 ± 0.08 | 1.40 ± 0.10 | 1.80 ± 0.10 |
| 4 | 2.9 ± 0.15 | 1.35 ± 0.09 | 1.85 ± 0.09 |
| 6 | 2.6 ± 0.20 | 1.35 ± 0.12 | 1.70 ± 0.10 |
| 9 | 2.5 ± 0.10 | 1.70 ± 0.20 | 1.50 ± 0.15 |
a
Cells were treated with 10 µM of daunorubicin (K562/R7 and HL60-MRP1 cells) in the presence or absence of 10 µM of steroid modulators and analysed by flow cytometry.
b
Cells were treated with 10 μM of mitoxantrone in presence or absence of 10 µM of steroid modulators and analysed by flow cytometry. All the results are expressed as fluorescence ratio with/without modulator.