Figure 6.

Schematic illustration of the design, construction, and application of light-up CMINs for monitoring of miR-34a replacement efficacy and accurate imaging-guided cancer therapy. ICG and miR-34a were simultaneously co-embedded in the nanocomplexes and subsequently stabilized by intermolecular cross-linking referred to as CMINs, which could better protect the embedded miR-34a and ICG and insure their release especially when dissociation of nanocomplexes happens. (A) Chemical structures of the components and preparation procedures of CMINs. (B) Schematic of the accumulation of CMINs in tumor sites via EPR effect (passive targeting). (C) Schematic of GSH-mediated dissociation of CMINs and the release of ICG and miR-34a upon intracellular uptake of nanocomplexes. Reproduced with permission from Ref. [109]. Copyright 2018 Wiley.