Table 1.

Characterization and validation.

Classification	Test	Result	Data
Morphology	Photography	Normal hiPSC morphology	Fig. 1 Panel C
Phenotype	Qualitative analysis	Immunohistochemistry of pluripotency markers: Oct4A, Nanog, Sox2, TRA-1-60, TRA-1-81	Fig. 1 Panel E
	Quantitative analysis	Expression of pluripotency marker Oct4 by flow cytometry	Fig. 1 Panel D
Genotype	Karyotype (G-banding) and resolution	Normal Karyotype: 46,XY, resolution: 480 band level	Fig. 1 Panel G
Identity	STR analysis	17 loci tested, 100% match	Submitted in archive with journal
Mutation analysis (IF	PCR and sequencing	Heterozygous targeted integration	Fig. 1 Panel F
APPLICABLE)	Southern Blot OR WGS	Not performed. Top 10 predicted coding and non-coding off-targets were sequenced, detecting no indel formation.	Available by request
Microbiology and virology	Mycoplasma	Mycoplasma testing by PCR: Negative	Supplementary Fig. 4
Differentiation potential	Embryoid body formation and Directed neural differentiation	Expression of genes from three germ layers following spontaneous differentiation: AFP, TBXT, MSI1 Directed neural differentiation immunohistochemistry: Human Nestin, Medium-length neurofilament polypeptide	Fig. 1 Panel H and I
Donor screening (OPTIONAL)	HIV 1 + 2 Hepatitis B, Hepatitis C	Not performed	N/A
Genotype additional info	Blood group genotyping	Not performed	N/A
(OPTIONAL)	HLA tissue typing	Not performed	N/A