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. 2018 Oct 3;121(1):105–114. doi: 10.1152/jn.00492.2018

Fig. 4.

Fig. 4.

5-Hydroxytryptamine (5-HT) increases spontaneous inhibition and tonic holding current on granule cells in a 5-HT2AR-dependent manner. A: schematic of the recording configuration. B: example whole cell, voltage-clamp recordings from 3 granule cells held at the reversal potential for excitation (0 mV) before and during bath application of 5-HT (left), with and without the 5-HT2A receptor antagonist MDL 100907 (middle) or SR95531 (right). C: mean normalized spontaneous inhibitory postsynaptic potential (sIPSCs) frequency across time in control (open circles, n = 13 granule cells treated with 5-HT, no MDL or gabazine present) and MDL (gray circles, n = 5 granule cells treated with 5-HT, MDL present; n = 8 granule cells treated with 5-HT, gabazine present) (left). Mean sIPSC frequency quantified at the time of peak 5-HT effect for individual granule cells (right). Solid black lines are mean and SE. D: mean normalized holding current across time in control (open circles, n = 9) and MDL (gray circles, n = 7) (left). Mean holding current quantified at the time of peak 5-HT effect for individual granule cells (right). Solid black lines are mean and SE. n.s., statistically nonsignificant; *P < 0.05.