Fig. 7.

5-Hydroxytryptamine (5-HT) reduces mossy fiber-evoked spike probability of granule cells in a 5 HT2A-dependent manner. A: schematic of the recording configuration. B: example traces from a cell-attached granule cell recording during electrical stimulation of mossy fibers and sequential bath application of 5-HT (10 μΜ), MDL 100907 (500 nM), gabazine (5 μM), and NBQX (5 μM). C: spike raster from the same example cell in B. D: mean spike probability for granule cells across conditions (n = 4). E: summary of mean spike number (left), mean first spike latency (middle), and spike jitter (right). n.s., statistically nonsignificant; *P < 0.05, **P < 0.01, ***P < 0.001.