Figure 8. Andrographolide increases DNA damage in PC3 cells.

Cells were incubated with Andrographolide 25 μM or vehicle (Control) for 24 hours. DNA damage was determined with a flow cytometry approach that detects cells that simultaneously express phosphorylated ATM and γH2AX. DNA damage is presented relative to Control cells, which is set at 1. Experiments were made in triplicate. Statistical analysis was performed using t-test. Mean ± SEM (^*P < 0.001).