Variables	Univariate analysis		Multivariate analysis
	HR (95% CI)	P value	HR (95% CI)	P value
Age (<60 years/≥60 years)	1.374 (1.002–1.884)	0.048
Gender (female/male)	0.958 (0.694–1.322)	0.794
Pathologic tumor status (T1+T2/T3)	3.876 (1.589–9.459)	0.003	2.891 (1.212–7.092)	0.020
Pathologic node status (N0/N1)	2.004 (1.336–3.005)	0.0008
Pathologic metastasis status (M0/M1)	2.047 (1.311–3.195)	0.002	1.629 (1.040–2.778)	0.033
Histological grade (G1/G2+G3)	1.488 (0.887–2.498)	0.132
Tumor margin status (negative/positive)	1.887 (1.363–2.613)	0.0001	1.629 (1.040–2.553)	0.002
Nerve plexus invasion (absence/presence)a	1.774 (1.246–2.525)	0.002
Lymphatic invasion (0, 1/2, 3)a	1.971 (1.340–2.899)	0.0006	1.768 (1.191–2.625)	0.005
Venous invasion (0, 1/2, 3)a	1.947 (1.305–2.907)	0.001
Intrapancreatic neural invasion (0, 1/2, 3)a	1.998 (1.379–2.894)	0.0003
Adjuvant chemotherapy (positive/negative)	0.981 (0.686–1.402)	0.915
Expression of CD3 (high/low)	1.629 (1.184–2.242)	0.003
Expression of CD4 (high/low)	1.606 (1.170–2.206)	0.003
Expression of CD8 (high/low)	1.847 (1.340–2.544)	0.0002	2.007 (1.450–2.778)	<0.0001
Expression of FOXP3 (high/low)	1.348 (0.983–1.848)	0.064
Ratio of FOXP3/CD4 (high/low)	0.883 (0.644–1.212)	0.442