Table 3. ROC curve parameters and 95% confidence intervals for GPC1 and GP2+GPC1 EV counts (EVs smaller than 150 nm only).

Parameter	AUC	95% CI	Cut-off value	Sensitivity %	95% CI	Specificity%	95% CI
GP2-positive EVs (EVs/uL)	0.5845	0.4405-0.7285	>10200.0	25.93	11.11-46.28	90.24	76.87-97.28
GPC1-positive EVs (EVs/uL)	0.6524	0.4972-0.8076	<972.0	25.93	11.11-46.28	95.45	77.16-99.88
GPC1+GP2-positive EVs (EVs/uL)	0.5237	0.3645-0.6829	<100.0	62.96	42.37-80.60	32.00	14.95-53.50