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. 2019 Feb 5;10(11):1171–1192. doi: 10.18632/oncotarget.26622

Figure 4. Effects of FGF2 knockdown on signaling pathway molecules and EMT marker proteins in the parental and pemetrexed-resistant lung cancer cells.

Figure 4

Figure 4

(A) Western blot analyses of expression of total or phosphorylated forms (pEGFR, pMEK, pERK, and pAKT) of signaling molecules in the PC9-MTA and H1993-MTA cells transfected with control siRNA (si-control) or siRNA targeting FGF2 (si-FGF2). β-actin was used as a loading control. The experiments were repeated independently at least three times, and one representative blot is provided in the figures. The quantitative numbers of relative expression levels corrected by β-actin were demonstrated below the picture of the blots. (B) Western blot analyses of expression of TS and EMT marker proteins in the PC9-MTA and H1993-MTA cells transfected with si-control or si-FGF2. (C) Morphological findings of PC9-MTA and H1993-MTA cells transfected with si-control or si-FGF2. Scale bars = 500 μm.