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. 2019 Feb 5;10(11):1171–1192. doi: 10.18632/oncotarget.26622

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Copyright: © 2019 Miura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A, B) Effects of PD173074 on the growth of pemetrexed-resistant PC9 PC9-MTA cells and H1993-MTA cells were measured by WST assay. The cells were cultured for 72 h in the presence of 0 to 5 μM PD173074. (C) Effects of PD173074 on the sensitivity to pemetrexed in the parental PC9 (P) and the PC9-MTA cells were measured by WST assay. (D) Effects of PD173074 on the sensitivity to pemetrexed in the parental H1993 (P) and H1993-MTA cells were measured by WST assay. The error bars in each graph represent the standard error of the value obtained in the experiments performed in triplicate. (E) The effects of PD173074 on the expression of total or phosphorylated forms of signaling molecules in the MAPK/ERK and PI3K/AKT pathways, EMT markers, and TS expression were analyzed by Western blotting in PC9-MTA and H1993-MTA cells. β-actin was used as a loading control. The experiments were repeated independently at least three times, and one representative blot is provided in the figures. The quantitative numbers of relative expression levels corrected by β-actin were demonstrated below the picture of the blots. (F) The effects of PD173074 on the expression of total or phosphorylated forms of signaling molecules in the MAPK/ERK and PI3K/AKT pathways, EMT markers, and TS expression were analyzed by Western blotting in PC9-MTA and H1993-MTA cells. β-actin was used as a loading control. The experiments were repeated independently at least three times, and one representative blot is provided in the figures. The quantitative numbers of relative expression levels corrected by β-actin were demonstrated below the picture of the blots.

(A, B) Effects of PD173074 on the growth of pemetrexed-resistant PC9 PC9-MTA cells and H1993-MTA cells were measured by WST assay. The cells were cultured for 72 h in the presence of 0 to 5 μM PD173074. (C) Effects of PD173074 on the sensitivity to pemetrexed in the parental PC9 (P) and the PC9-MTA cells were measured by WST assay. (D) Effects of PD173074 on the sensitivity to pemetrexed in the parental H1993 (P) and H1993-MTA cells were measured by WST assay. The error bars in each graph represent the standard error of the value obtained in the experiments performed in triplicate. (E) The effects of PD173074 on the expression of total or phosphorylated forms of signaling molecules in the MAPK/ERK and PI3K/AKT pathways, EMT markers, and TS expression were analyzed by Western blotting in PC9-MTA and H1993-MTA cells. β-actin was used as a loading control. The experiments were repeated independently at least three times, and one representative blot is provided in the figures. The quantitative numbers of relative expression levels corrected by β-actin were demonstrated below the picture of the blots. (F) The effects of PD173074 on the expression of total or phosphorylated forms of signaling molecules in the MAPK/ERK and PI3K/AKT pathways, EMT markers, and TS expression were analyzed by Western blotting in PC9-MTA and H1993-MTA cells. β-actin was used as a loading control. The experiments were repeated independently at least three times, and one representative blot is provided in the figures. The quantitative numbers of relative expression levels corrected by β-actin were demonstrated below the picture of the blots.