Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 5;10(11):1171–1192. doi: 10.18632/oncotarget.26622

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © 2019 Miura et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

(A, B) ZEB1 expression was quantitated by real-time RT-PCR in the parental PC9 cells (P) and PC9-MTA cells (A) and the parental H1993 (P) cells and H1993-MTA cells (B) transfected with control si-RNA (si-control) and siRNA targeting ZEB1 (si-ZEB1). (C, D) Sensitivity to pemetrexed was measured in parental (P) and PC9-MTA cells (C) and in parental H1993 (P) and H1993-MTA cells (D) transfected with each si-RNA (si-control and si-ZEB1) using the WST assay. The error bars in each graph represent the standard error of the value obtained in the experiments performed in triplicate. (E) Western blot analysis of the expression of EMT marker proteins in PC9-MTA and H1993-MTA cells transfected with si-control or si-ZEB1. β-actin was used as a loading control.

(A, B) ZEB1 expression was quantitated by real-time RT-PCR in the parental PC9 cells (P) and PC9-MTA cells (A) and the parental H1993 (P) cells and H1993-MTA cells (B) transfected with control si-RNA (si-control) and siRNA targeting ZEB1 (si-ZEB1). (C, D) Sensitivity to pemetrexed was measured in parental (P) and PC9-MTA cells (C) and in parental H1993 (P) and H1993-MTA cells (D) transfected with each si-RNA (si-control and si-ZEB1) using the WST assay. The error bars in each graph represent the standard error of the value obtained in the experiments performed in triplicate. (E) Western blot analysis of the expression of EMT marker proteins in PC9-MTA and H1993-MTA cells transfected with si-control or si-ZEB1. β-actin was used as a loading control.