Table 2. Plasmids used in transient transfection for luciferase assay.
| Construct | Amount | Purpose |
|---|---|---|
| pGL3-test (KP,KU,KPU,KPUI) | 800 ng | Luciferase assay |
| pGL3-basic | 800 ng | Luciferase assay (internal control) |
| pRL-TK (Renilla) | 30 ng | Luciferase assay (internal control) |
| pGL3-KPU | 800 ng | Forced assay |
| pGL3-SP | 800 ng | Forced assay |
| pCMV6_Elk1 | 300 ng | Forced assay |
| pCMV6_Elk1-3R | 300 ng | Forced assay |
| pCMV6-Myc (empty) | 300 ng | Forced assay |
pGL3-test represents pGL3 vector containing four different constructs (KP:KATNA1 Promoter, KU: KATNA1 5’ UTR, KPU: KATNA1 Promoter + 5’ UTR, KPUI: KATNA1). SP represents SPG4 promoter.