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. 2019 Feb 21;14(2):e0212518. doi: 10.1371/journal.pone.0212518

Table 4. Primer sequences for site directed mutagenesis.

Primer Sequence
K230R_F 5’ CGAGGCCCCAAACCTGAGATCGGAAGAGCTTAATG 3’
K230R_R 5’ CATTAAGCTCTTCCGATCTCAGGTTTGGGGCCTCG 3’
K249R_F 5’ CTTTGCCCCCAGAAGTGAGAGTAGAAGGGCCC 3’
K249R_R 5’ GGGCCCTTCTACTCTCACTTCTGGGGGCAAAG 3’
K254R_F 5’TGAAAGTAGAAGGGCCCAGGGAAGAGTTGGAAGT 3’
K254R_R 5’ ACTTCCAACTCTTCCCTGGGCCCTTCTACTTTCA 3’

F: Forward primer, R: Reverse primer.