Table 4. Primer sequences for site directed mutagenesis.
Primer | Sequence |
---|---|
K230R_F | 5’ CGAGGCCCCAAACCTGAGATCGGAAGAGCTTAATG 3’ |
K230R_R | 5’ CATTAAGCTCTTCCGATCTCAGGTTTGGGGCCTCG 3’ |
K249R_F | 5’ CTTTGCCCCCAGAAGTGAGAGTAGAAGGGCCC 3’ |
K249R_R | 5’ GGGCCCTTCTACTCTCACTTCTGGGGGCAAAG 3’ |
K254R_F | 5’TGAAAGTAGAAGGGCCCAGGGAAGAGTTGGAAGT 3’ |
K254R_R | 5’ ACTTCCAACTCTTCCCTGGGCCCTTCTACTTTCA 3’ |
F: Forward primer, R: Reverse primer.