Table 5. KATNA1 oligonucleotides used in EMSA.
Oligo name | Sequence |
---|---|
Promoter_WT | 5’ AGGGTGGAGATTGAGACTGGAGGAAGCCCTGTGCAGTACATAT 3’ |
UTR1_WT | 5’ CGAGGTCGTCCCCGGCACCGGAAGTGACCCTGGCGGGTTTGT 3’ |
UTR2_WT | 5’ GACTGGGTCAGGCCCTCCTTCCTCGCTGCCGGGATCTCCACTC 3’ |
Promoter_Mut | 5' AGGGTGGAGATTGAGACCAAGAAGGACCCTGTGCAGTACATAT 3' |
UTR1_Mut | 5' CGAGGTCGTCCCCGGTGTTAAGGATGACCCTGGCGGGTTTGT 3' |
UTR2_Mut | 5' GACTGGGTCAGGCCCTCTCCTTCTATTGCCGGGATCTCCACTC-3' |
Elk1 binding sites are shown in bold. WT represents wild-type. Purine-purine and pyrimidine-pyrimidine converted oligonucleotides in Elk1 binding sites are named as Mut.