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. 2019 Feb 8;15(2):e1007905. doi: 10.1371/journal.pgen.1007905

Fig 2. High 22G RNA levels are associated with low mRNA expression, compatible with silencing.

Fig 2

A) Mean normalized mRNA [55] and 22G RNA reads detected in hermaphrodite and male gonads (data in 20 bins according to 22G RNA level; number of genes per bin: 4–1101 in hermaphrodites, 5–1343 in males; mean: solid line, outliers: circles). mRNA expression is globally decreased for the top 5% of genes (shaded area) with high levels of 22G RNA. See S3A Fig and S3B Fig for statistical analysis. B) mRNA expression for WAGO-target genes and non-target genes (Bonferroni corrected p-values of the Wilcoxon rank sum test q<0.01 indicated by stars, ns = not significant, na = not applicable). Data are the same as for Fig 2A but plotted as WAGO targets [20] and non-WAGO targets in hermaphrodites and males. WAGO targets were available only for hermaphrodites and interpolated to male samples. C) mRNA expression for CSR-1-target genes and non-target genes (Bonferroni corrected p-values of the Wilcoxon rank sum test q<0.01 indicated by stars, ns = not significant, na = not applicable). Data are the same as for Fig 2A but plotted as CSR-1 targets and non-CSR-1 targets in hermaphrodite and male [35,56]. CSR-1 targets were available for each sex and analyzed separately.