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. 2019 Feb 21;8:e41563. doi: 10.7554/eLife.41563

Figure 1. Knockdown of ephrin-B3 does not alter synapse density in single-neuron microislands.

(a and b) Representative images of dendrites from single-neuron microislands transduced with control (pSuper) and eB3 shRNA viruses and immunostained at DIV21 for vGlut1 and PSD-95. Scale bar, 20 μm. (c) Higher magnification images of boxed regions shown in a and b. Arrowheads indicate colocalized vGlut1 and PSD-95 puncta. Scale bar, 5 μm. (d) Quantification of synapse density in single neuron microislands (Control, n=27; eB3 shRNA, n=47; t(72)=0.9390, p=0.3509, two-tailed Student’s t-test) transduced with the indicated viruses.

Figure 1—source data 1. Knockdown of ephrin-B3 does not alter synapse density in single-neuron microislands.
elife-41563-fig1-data1.xlsx (10.8KB, xlsx)
DOI: 10.7554/eLife.41563.003

Figure 1.

Figure 1—figure supplement 1. Schematic of microisland experimental design.

Figure 1—figure supplement 1.

(a) Single-neuron microislands provide a system devoid of potential competitive interactions. Blue dots represent endogenous PSD-95, green dots represent PSD-95-GFP (b) Two-neurons microislands allow for an assessment of direct competition for synapses between individual neurons. Black dots represent synapses.