Figure 2. PSD-95-GFP localizes to synapses and does not alter synapse density.

(a and b) Representative images of single-neuron microislands transfected with the indicated constructs and immunostained for GFP (for PSD-95-GFP), PSD-95 and vGlut1. Boxed regions are shown in enlarged insets. Scale bars represent 20 μm in merged images and 5 μm in insets. (c) Comparison of synapse density quantification in single neuron microislands using PSD-95 or GFP immunostaining for PSD-95-GFP (n = 18 cells; t(34)=0.08148, p=0.9355, two-tailed Student’s t-test). (d) Quantification of synapse density in single neurons transfected with the indicated constructs using PSD-95 and vGlut1 immunostaining (Untransfected, n = 20; PSD-95-GFP, n = 18; PSD-95-GFP + eB3 shRNA, n = 18; F(2,53)=.1008, p=0.9043, one-way ANOVA).