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. 2019 Feb 21;8:e41563. doi: 10.7554/eLife.41563

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Genetic reagent (Mus musculus) ephrin-B3 knockout (and WT littermates) Yokoyama et al., 2001, Hruska et al., 2015, Antion et al., 2010. RRID:MGI:3026744
Genetic reagent (M. musculus) MADM-11 TG, eB3+/MADM-11 TG, eB3- (founder line) This paper. Progenitor: MADM-11 TG/TG (Hippenmeyer et al., 2010), RRID: IMSR_JAX:013749
Genetic reagent (M. musculus) MADM-11 GT/GT; Emx1-Cre Hippenmeyer et al., 2010, Beattie et al., 2017. Progenitor: IMSR_JAX:005628
Genetic reagent (M. musculus) MADM-11 GT/GT; Nestin-spCre Hippenmeyer et al., 2010.
Strain, strain background (Rattus norvegicus domesticus) Long Evans Rat Charles River Strain code: 006: RRID:RGD_2308852
Aantibody Rabbit polyclonal anti-GFP Thermo Fisher Scientific A6455, RRID:AB_221570 (1:2500)
Aantibody Mouse monoclonal anti-PSD-95 NeuroMab (clone 28/43) Cat. # 75–028, RRID:AB_2292909 (1:500)
Antibody Guinea pig polyclonal anti-vGlut1 Millipore Cat. #AB5905, RRID:AB_2301751 (1:5000)
Antibody Rabbit polyclonal anti-RFP Rockland antibodies Cat. # 600-401-379, RRID:AB_2209751 (1:500)
Antibody Chicken polyclonal anti-GFP Abcam Cat. # ab13970, RRID:AB_300798 (1:500)
Antibody Rat monoclonal anti-CTIP2 Abcam Cat. # ab18465, RRID:AB_10015215 (1:500)
Antibody Mouse monoclonal anti-SATB2 Abcam Cat. # ab51502, RRID:AB_882455 (1:500)
Antibody Donkey polyclonal anti-rabbit Dylight 488 Abcam Cat. # ab96919, RRID:AB_10679362 (1:500)
Antibody Donkey polyclonal anti-chicken Dylight 488 Jackson Immunoresearch Cat. # ab96947, RRID:AB_10681017 (1:500)
Antibody Donkey polyclonal anti-rabbit Alexafluor 594 Jackson Immunoresearch Cat. # 711-585-152, RRID:AB_2340621 (1:500)
Antibody Donkey polyclonal anti-rat 647 Jackson Immunoresearch Cat. # 712-605-153, RRID:AB_2340694 (1:500)
Antibody Goat polyclonal anti-mouse ATTO 425 Rockland antibodies Cat. # 611-151-122, RRID:AB_10893217 (1:250)
Antibody Donkey polyclonal anti-guinea pig Dylight 649 Jackson Immunoresearch Cat. # 706-605-148, RRID:AB_2340476 (1:500)
Recombinant DNA reagent pFUGW-tdTomato_H1-eB3.2 (plasmid) Hruska et al., 2015. Progenitor: pFUGW (RRID:Addgene_14883); pSuper.
Recombinant DNA reagent pFUGW-tdTomato_H1-pSuper (plasmid) Hruska et al., 2015. Progenitor: pFUGW (RRID:Addgene_14883), pSuper.
Recombinant DNA reagent pSuper (plasmid) Hruska et al., 2015, McClelland et al., 2010.
Recombinant DNA reagent eB3.2 shRNA (plasmid) Hruska et al., 2015, McClelland et al., 2010. pSuper
Recombinant DNA reagent pFUGW (plasmid) Hruska et al., 2015, McClelland et al., 2010. RRID:Addgene_14883
Recombinant DNA reagent pLL3.7 (plasmid) Addgene RRID:Addgene_11795
Recombinant DNA reagent ephrin-B3 shRNA lentivirus Penn Vector Core (University of Pennsylvania) Progenitor: pLL3.7 vector (RRID:Addgene_11795)
Peptide, recombinant protein Recombinant human EphB2 Fc chimera R and D systems Cat. # 5189-B2
Peptide, recombinant protein Recombinant Fc control fragment R and D systems 110-HG-100
Commercial assay or kit RNAscope fluorescent multiplex kit Advanced Cell Diagnostics 320850
Commercial assay or kit RNAscope probe- Mm-Bcl11b (CTIP2) Advanced Cell Diagnostics Cat. # 413271-C3
Commercial assay or kit RNAscope probe- Mm-Satb2-C2 Advanced Cell Diagnostics Cat. # 413261-C2
Commercial assay or kit RNAscope probe- Mm-Efnb3 Advanced Cell Diagnostics Cat. # 526771
Chemical compound, drug Tetrodotoxin (TTX) Tocris Cat. #1078
Software, algorithm NIH ImageJ McClelland et al., 2009, Hruska et al., 2015. RRID:SCR_003070