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. 2019 Feb 8;8:e43125. doi: 10.7554/eLife.43125

Figure 4. zGrad-mediated depletion of Cadherin-1 at 25 hpf results in skin defects and lethality.

(A) Breeding strategy to assess heat shock-induced zGrad-mediated degradation of Cdh1-sfGFP on embryonic development. (B, C, D) Images of cdh1+/-; hsp70l:zgrad/+; cdh1:cdh1-sfGFP/+ control embryo (B), cdh1-/-; cdh1:cdh1-sfGFP/+ control embryo (C) and cdh1-/-; hsp70l:zGrad/+; cdh1:cdh1-sfGFP embryo with skin defects (D) at 29 hpf. Embryos were heat shocked at 25 hpf for 1 hr. Scale bar: 200 µm. Insets in (C, D) are magnified images of the skin in the same embryos. Scale bar: 50 µm.

Figure 4.

Figure 4—figure supplement 1. Expected results for zGrad-induced depletion of sfGFP-tagged Cadherin-1.

Figure 4—figure supplement 1.

(A) Crossing scheme of indicated genotypes and % of embryos with expected and observed phenotype.