Table 2. Summary of degradation kinetics.
| FP-Tagged protein | Promoter expressing zGrad | Percent reduction | Degradation half-life in min | Time for onset of degradation in min |
|---|---|---|---|---|
| sfGFP-ZF1 | mRNA | 89% | N/A | N/A |
| Cxcr4b-EGFP | cxcr4b | 86% | N/A | N/A |
| H2A-EGFP (in somites) |
hsp70l | 87% | 156 | 200 |
| H2A-EGFP (in primordium) |
hsp70l | 22% | 29† | 140 |
| Cdh1-sfGFP | hsp70l | 79% | 24 | 75 |
| Ctnna-Citrine | hsp70l | 58% | 19 | 65 |
†Note that the zGrad-mediated degradation of H2A-EGFP in the primordium is obscured by the continued production of H2A-EGFP.