Figure 4. Absence of leptin signaling in early life causes insulin resistance.

(A–B) Glucose tolerance test (GTT; 2 g glucose/kg b.w.; i.p.) and the area under the curve (AUC) of the GTT in male mice (n = 10–32). (C–D) Insulin tolerance test (ITT; 1 IU/kg b.w.; i.p.) and the AUC of the ITT in male mice (n = 7–27). (E) Serum insulin concentration in male mice (n = 10–25). (F–G) GTT and the AUC of the GTT in female mice (n = 6–13). (H–I) ITT and the AUC of the ITT in female mice (n = 6–12). (J) Serum insulin concentration in female mice (n = 8–14). *p<0.05 vs Ubi group; **p<0.01 vs Ubi group; ***p<0.001 vs Ubi group; ****p<0.0001 vs Ubi group. # p<0.05 vs LepRNull group; ## p<0.01 vs LepRNull group; ### p<0.001 vs LepRNull group; #### p<0.0001 vs LepRNull group (Figure 4—source data 1).