Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) |
STOCK Leprtm1Jke/J | The Jackson Laboratory |
JAX:018989 | |
| Genetic reagent (M. musculus) |
B6.Cg-Ndor1Tg(UBC-cre/ERT2)1Ejb/2J | The Jackson Laboratory |
JAX:008085 | |
| Genetic reagent (M. musculus) |
B6.Cg-Lepob/J | The Jackson Laboratory |
JAX:000632 | |
| Genetic reagent (M. musculus) |
B6.129-Leprtm2(cre)Rck/J | The Jackson Laboratory |
JAX:008320 | |
| Genetic reagent (M. musculus) |
B6;129S6-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J |
The Jackson Laboratory |
JAX:007909 | |
| Antibody | Rabbit anti-GnRH (LHRH) antibody |
Immunostar | RRID:AB_572248 | IF (1:2000) |
| Antibody | Rabbit anti-pSTAT3Tyr705 antibody | Cell Signaling | Cat. #: 9131 | IHC (1:1000) |
| Antibody | Mouse anti-αMSH antibody |
Chemicon | Cat. #: AB5087 | IF (1:2000) |
| Antibody | Rabbit anti-βendorphin antibody |
Phoenix Pharmaceuticals |
Cat. #: H-022–33 | IF (1:2000) |
| Antibody | Rabbit anti-AgRP antibody |
Phoenix Pharmaceuticals |
Cat. #: H-003–53 | IF (1:2000) |
| Antibody | Biotin-SP-conjugated AffiniPure Donkey anti-Rabbit IgG |
Jackson ImmunoResearch |
Cat. #: 711-065-152 | IHC (1:1000) |
| Antibody | Alexa Fluor488-conjugated Donkey anti-Rabbit IgG |
Jackson ImmunoResearch | Cat. #: 711-545-152 | IF (1:500) |
| Antibody | Alexa Fluor594-conjugated Donkey anti-Mouse IgG |
Jackson ImmunoResearch | Cat. #: 715-585-150 | IF (1:500) |
| Sequence- based reagent |
RT-qPCR primers | This paper | ||
| Peptide, recombinant protein |
Human recombinant insulin |
Novo Nordisk | ||
| Peptide, recombinant protein |
Mouse recombinat leptin | National Hormone and Peptide Program |
||
| Commercial assay or kit |
Mouse Leptin ELISA KIT | Crystal Chem | 90030 | |
| Commercial assay or kit |
Total Rat/Mouse T4 ELISA KIT | Calbiotech | T4044T-100 | |
| Commercial assay or kit |
Rat/Mouse Testosterone ELISA KIT |
Calbiotech | TE187S-100 | |
| Commercial assay or kit |
Mouse Insulin ELISA KIT | Crystal Chem | 90080 | |
| Commercial assay or kit |
Corticosterone EIA KIT | Arbor Assays | K014-H1 | |
| Chemical compound, drug |
Tamoxifen | Sigma-Aldrich | T5648 | |
| Chemical compound, drug |
Sesame oil | Sigma-Aldrich | S3547 | |
| Software, algorithm | ImageJ | National Institutes of Health (NIH) | http://rsb.info.nih.gov/ij/ | |
| Software, algorithm | Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/ | Version 6 |
| Software, algorithm | ANY-maze | ANY-maze | http://anymaze.co.uk/ | |
| Other | High-fat diet (HFD) 5.31 kcal/g, 58% calories from fat |
Pragsoluções |