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. 2019 Feb 19;93(5):e01635-18. doi: 10.1128/JVI.01635-18

FIG 3.

FIG 3

Subcellular localization of TGBp3 and dsRNA in PVX infected N. benthamiana cells. (A) Confocal micrographs of dsRNA (red) and TGBp3-YFP (green) in N. benthamiana leaf tissue during PVX infection at 48 hpi. TGBp3-YFP was expressed from pGR107/TGBp3-YFP, and the dsRNA was labeled using the mRFP-based dRBFC assay. The white arrowheads indicate TGBp3 foci at the cell periphery. Scale bar = 50 μm (I to III) or 10 μm (IV to IX). (B) TGBp3-mRFP is not associated with dsRNA in the absence of PVX infection. Scale bar = 10 μm. (C) Confocal micrographs of TGBp3-CFP (cyan), YFP-TGBp2 (green), and dsRNA (red) in N. benthamiana epidermal cells during PVX infection at 48 hpi. YFP-TGBp2 was expressed from pGR107/YFP-TGBp2. Scar bar = 10 μm.