FIG 6.

The central and C-terminal hydrophilic domains of TGBp2 are required for interactions with viral RdRp. (A) Illustration of TGBp2 mutants. The two transmembrane motifs (TM1 and TM2) are shown in black. (B) BiFC for protein-protein interactions between RepN and TGBp2 mutants in N. benthamiana epidermal cells at 48 hpi. Micrographs were obtained using the same parameters. Scale bars = 50 μm. (C) MYTH for protein-protein interactions between RepN and TGBp2 mutants. (D) Subcellular localization of TGBp2 mutants. The insets show the typical morphology of ER-derived vesicles induced by TGBp2 or its mutants. Scare bar = 50 μm. (E) Subcellular localization of dsRNA and TGBp2 mutants during PVX infection. The nuclei are indicated by white asterisks. Scale bar = 50 μm.