FIG 3.

EBV-transformed B cells are retained by CHO cells that express MAdCAM-1. (A) CHO cells were stably transfected with pcDNA3.1 vectors expressing MAdCAM-1 or EBV gp350 or with the pcDNA3.1 parental vector. These cell lines were incubated with 4 independent EBV-transformed B cell samples. The dot plot shows the percentage of B cells adherent to the stably transfected CHO clones. (B) Lymphocyte adhesion to CHO cells that stably express MAdCAM-1 was blocked by an antibody directed against LPAM-1 (Act-1). An antibody of the same class and type but without target specificity served as an isotype control (IC). Three replicates of two independent LCLs were analyzed, and the results are shown in the dot plot (n = 6).