FIG 1.

Run-on oligomer (ROO) filament of SgrAI/DNA complexes and sites of point mutations. (A) The run-on oligomer filament structure with 11 SgrAI/DNA complexes, each colored separately. The red box indicates the area shown in panels E and F. (B) Cartoon and surface rendering of one SgrAI/DNA complex in the same orientation as the salmon-colored complex in panel A. (C) View rotated 180° about a vertical axis relative to that in panel B. (D) View rotated 90° from that in panel C, showing the same orientation as the lightest blue-colored complex in panel A. (E) Zoom-in on the boxed area of panel A, showing the interface between two adjacent SgrAI/DNA complexes within the run-on oligomer filament. Selected residues mutated in this study are indicated. (F) Approximately 90° rotation from panel B, showing the positions of selected residues mutated in this study.