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DNA sequences used in the single-turnover DNA cleavage assays
| Name | Sequencea |
|---|---|
| PC-top | 5′-GATGCGTGGGTCTTCACA-3′ |
| PC-bot | 3′-CTACGCACCCAGAAGTGTGGCC-5′ |
| 18-1-top | 5′-AAGTCCA|CCGGTGGACTT-3′ |
| 18-1-bot | 3′-TTCAGGTGGCC|ACCTGAA-5′ |
Recognition sequences are shown in boldface; a vertical line (|) marks the SgrAI-specific cleavage site.
