FIG 5.
Effects of 2Apro mutations on virus recovery. RNA transcripts were generated from infectious clones of the wt or VP1-I194V in the presence or absence of the 2Apro mutations 2A-I99V/-G102R, transfected into mouse L cells or HeLa cells, and incubated at 37°C for 24 h. (A) Virus titers recovered from transfected mouse L cells (n = 3; error bars show SEM; ***, P < 0.0001) (B) Virus titers recovered from transfected HeLa cells. (n = 3; error bars show SEM; ***, P < 0.0001) (C) Virus samples recovered from HeLa cells were diluted in serum-free medium to equal starting titers, incubated at a range of temperatures between 37°C and 55°C for 30 min, cooled to 4°C, and titrated by plaque assays using HeLa cells (n = 2; error bars show standard deviations [SD]; *, P < 0.05 compared to wt).