FIG 4.

Rotavirus gene 7 determines the ES phenotype. (A) RNA purified from MA104 cells infected (9 h, MOI of 10) with the different monoreassortants and parental strains was subjected to RT-PCR with XBP1 and GAPDH primers. The PCR DNA products were analyzed by electrophoresis and ethidium bromide staining on two agarose gels (middle and lower panels). The sizes of the molecular weight markers (MW) in base pairs are indicated on the left. (B) RNA purified from mock-infected MA104 cells (lane 5) or cells infected with either an RRV monoreassortant carrying gene 7 from RF (RRV/RF07 lane 2), an RF monoreassortant carrying gene 7 from RRV (RF/RRV07 lane 3), or the RRV (lane 1) or RF (lane 4) parental strains was subjected to RT-PCR with XBP1 and GAPDH primers. The PCR DNA products were analyzed by electrophoresis and ethidium bromide staining on two agarose gels. The sizes of the molecular weight markers (MW) in base pairs are indicated on the left.