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ES phenotypes of recombinant viruses bearing NSP3 chimeras The DNA products obtained by RT-PCR (with XBP1 or GAPDH primers) of RNA extracted from mock-infected cells or from cells infected (MOI of 10 [9 hpi]) with RF or RRV or a recombinant virus (SA11 background, bearing RRV-247-RF or RF-247-RRV gene 7 chimeras) were analyzed by agarose gel electrophoresis and ethidium bromide staining, together with the GAPDH PCR control.
